Tyrosinase Activity Colorimetric Assay

Tyrosinase is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway: first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin. Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments.

Tribioscience’s Tyrosinase Activity Assay Kit provides a simple, sensitive assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with a probe forming a highly stable chromophore with absorbance at 510 nm. The assay can detect as low as 30 µU Tyrosinase in biological samples.

Assay Principle

Application

• Measurement of Tyrosinase activity in various biological samples.
• Analysis of Tyrosinase in pathological conditions.

 

Diagram for Tyrosinase Standard Preparation

Standard Curve

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Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)