TruSight Oncology 500 v2 DNA Automation Kit, For Use with NextSeq 550 (64 samples)
Vendor: Illumina
SKU: 20130543
In stock
Includes reagents for preparing and enriching up to 64 DNA samples, and NextSeq 500/550 sequencing reagents. Purchase indexes separately. Intended for use with automation.
TruSight Oncology 500 v2 is a pan-cancer next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from formalin-fixed, paraffin-embedded (FFPE) tissue for oncology research.
Documents
- Analysis of TMB and MSI Status with TruSight Oncology 500
- Input Recommendations for TMB, MSI, and Small Variant Analysis with TruSight Oncology 500
- TruSight Oncology 500 Gene List - English
- TruSight Oncology 500 Gene List - Chinese (Simplified)
- TruSight Oncology 500 and TruSight Oncology 500 High-Throughput
- Data analysis and reporting for the TruSight Oncology 500 portfolio
- Chinese (Simplified)
- TruSight Oncology 500 portfolio
Support Documentation
Specifications
- Area of interest Cancer research, Drug discovery & development, Immuno-oncology research, Immunology
- Assay time 3–4 days from sample input to final results
- Automation capability Liquid handling robot(s)
- Cancer type Pan-cancer, Solid tumor
- Content specifications Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included. The included HRD panel † includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics. † TruSight Oncology 500 v2 is not available for sale in Japan
- Hands on time ~3.25 hrs for automated workflow ~5-7 hrs for manual workflow
- Input quantity 30 ng DNA (as low as 10 ng), 40 ng RNA (as low as 20 ng)
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research ModeNovaSeq 6000 SystemNovaSeq 6000Dx in Research ModeNovaSeq X Plus SystemNovaSeq X System
- Key use Targeted analysis, Tumor immunogenicity analysis
- Method Target enrichment, Targeted DNA sequencing, Targeted RNA sequencing
- Multiplexing NextSeq 550/Dx: 8 samples/run. NexSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples. NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples. NovaSeq X/X+: 1.5 B 32 samples, 10B 192 samples, 25B 480 samples.
- Nucleic acid type DNA, RNA
- Specialized sample types FFPE tissue
- Species category Human
- Technology Sequencing
- Variant class Copy number variants (CNVs), Gene fusions, Genomic instability score (GIS), Insertions-deletions (indels), Loss of heterozygosity (LOH), Microsatellite instability (MSI), Multi-nucleotide variants (MNVs), Single nucleotide variants (SNVs), Somatic variants, Transcript variants, Tumor mutational burden (TMB)
links and documants
- Datasheet url Click here
- Source url Click here
Couldn't load pickup availability
TruSight Oncology 500 v2 DNA Automation Kit, For Use with NextSeq 550 (64 samples)
Quick order list
Free shipping on almost everything
Free Shipping
All orders over $500 are delivered to your doorstep at no extra charge.
30-Days Free Returns
Enjoy the freedom of stress-free shopping with our hassle-free and return policy.
Secure Payment
Shop with confidence knowing your payments are secure and your information