TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples)
Vendor: Illumina
SKU: 20049282
In stock
Includes reagents for preparing and enriching up to 32 DNA and RNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
TruSight Oncology 500 High-Throughput (HT) is a highly scalable pan-cancer, next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from FFPE tissue.
Documents
- TruSight Oncology 500 Gene List - English
- TruSight Oncology 500 Gene List - Chinese (Simplified)
- TruSight Oncology 500 and TruSight Oncology 500 High-Throughput
- Data analysis and reporting for the TruSight Oncology 500 portfolio
- Chinese (Simplified)
- TruSight Oncology 500 portfolio
- Brazilian Portuguese
- Automating TruSight Oncology 500 High-Throughput
Support Documentation
- DRAGEN TruSight Oncology 500 Analysis Software v1.1 on ICA Documentation
- DRAGEN TruSight Oncology 500 Analysis Software v2.1 Documentation
- DRAGEN TruSight Oncology 500 Analysis Software v2.1.2 on ICA Documentation
- TruSight Oncology 500 v1.0 Local App Documentation
- DRAGEN TruSight Oncology 500 Analysis Software v1.1 Documentation
- TruSight Oncology 500 High Throughput Reference Guide Documentation
- DRAGEN TruSight Oncology 500 Analysis Software v2.5 Documentation
- TruSight Oncology 500 v2.0 Local App Documentation
Specifications
- Area of interest Cancer research, Drug discovery & development, Immuno-oncology research, Immunology
- Assay time 4–5 days from sample to results
- Automation capability Liquid handling robot(s)
- Cancer type Pan-cancer, Solid tumor
- Content specifications Targeted sequencing of DNA from 523 genes of interest and RNA from 55 genes, for a total of 1.94 Mb panel size. MSI and TMB measurement included.
- Hands on time ~2.5 hr for automated workflow ~10.5 hr for manual workflow
- Input quantity 40 ng DNA and/or 40–80 ng RNA
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNovaSeq 6000 SystemNovaSeq 6000Dx in Research ModeNovaSeq X Plus SystemNovaSeq X System
- Key use Targeted analysis, Tumor immunogenicity analysis
- Method Target enrichment, Targeted DNA sequencing, Targeted RNA sequencing
- Multiplexing NextSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples. NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples. NovaSeq X Series*: 1.5B cartridge 32 samples, 10B cartridge 192 samples, 25B 480 samples.
- Nucleic acid type DNA, RNA
- Specialized sample types FFPE tissue
- Species category Human
- Technology Sequencing
- Variant class Copy number variants (CNVs), Gene fusions, Insertions-deletions (indels), Novel transcripts, Single nucleotide variants (SNVs), Somatic variants, Structural variants, Transcript variants
links and documants
- Datasheet url Click here
- Source url Click here
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TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples)
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