TruSight Oncology 500 v2 DNA/RNA Kit (24 samples) 20130527 - Illumina

TruSight Oncology 500 DNA/RNA Automation Kit plus Velsera interpretation report, For Use with NextSeq (16 indexes, 32 Samples)

Name: TruSight Oncology 500 DNA/RNA Automation Kit plus Velsera interpretation report, For Use with NextSeq (16 indexes, 32 Samples)
Brand: Illumina
SKU: 20045991
Availability: InStock

Vendor: Illumina

SKU: 20045991

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Includes reagents for preparing and enriching up to 32 DNA and RNA samples and 16 indexes, data interpretation reports using Pierian Clinical Genomics Workspace, and NextSeq 500/550 sequencing reagents. Intended for use with automation.

TruSight Oncology 500 v2 is a pan-cancer next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from formalin-fixed, paraffin-embedded (FFPE) tissue for oncology research.

Documents

Support Documentation

Specifications

Area of interest Cancer research, Drug discovery & development, Immuno-oncology research, Immunology
Assay time 3–4 days from sample input to final results
Automation capability Liquid handling robot(s)
Cancer type Pan-cancer, Solid tumor
Content specifications Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included. The included HRD panel † includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics. † TruSight Oncology 500 v2 is not available for sale in Japan
Hands on time ~3.25 hrs for automated workflow ~5-7 hrs for manual workflow
Input quantity 30 ng DNA (as low as 10 ng), 40 ng RNA (as low as 20 ng)
Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research ModeNovaSeq 6000 SystemNovaSeq 6000Dx in Research ModeNovaSeq X Plus SystemNovaSeq X System
Key use Targeted analysis, Tumor immunogenicity analysis
Method Target enrichment, Targeted DNA sequencing, Targeted RNA sequencing
Multiplexing NextSeq 550/Dx: 8 samples/run. NexSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples. NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples. NovaSeq X/X+: 1.5 B 32 samples, 10B 192 samples, 25B 480 samples.
Nucleic acid type DNA, RNA
Specialized sample types FFPE tissue
Species category Human
Technology Sequencing
Variant class Copy number variants (CNVs), Gene fusions, Genomic instability score (GIS), Insertions-deletions (indels), Loss of heterozygosity (LOH), Microsatellite instability (MSI), Multi-nucleotide variants (MNVs), Single nucleotide variants (SNVs), Somatic variants, Transcript variants, Tumor mutational burden (TMB)

Components

20045490 — TruSight Oncology RNA Lib.Prep Auto
20045491 — TruSight Oncology Lib. Prep -20C Auto
20045492 — TruSight Oncology Lib. Prep 4C Auto
20006937 — TruSight Oncology UP Index Primers
20045493 — TruSight Oncology Enrichment -20C Auto
20045494 — TruSight Oncology Enrichment 4C Auto
20045495 — TruSight Oncology 500DNA/RNA ProbesAuto
15057929 — NextSeq 500 High output reagent Carr.V2 (300 cyc)
20022408 — NextSeq High Output Flow Cell V2.5
15057941 — NextSeq 500 buffer cartrige v2
15058251 — NextSeq accessory box V2

links and documants

Datasheet url Click here
Source url Click here