TruSight Oncology 500 DNA Kit for Use with NextSeq plus Velsera interpretation report (16 indexes, 48 Samples)
Vendor: Illumina
SKU: 20032625
In stock
Includes reagents for preparing and enriching up to 48 DNA samples and 16 indexes, data interpretation reports using Pierian Clinical Genomics Workspace, and NextSeq 500/550 sequencing reagents.
TruSight Oncology 500 v2 is a pan-cancer next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from formalin-fixed, paraffin-embedded (FFPE) tissue for oncology research.
Documents
- Analysis of TMB and MSI Status with TruSight Oncology 500
- Input Recommendations for TMB, MSI, and Small Variant Analysis with TruSight Oncology 500
- TruSight Oncology 500 Gene List - English
- TruSight Oncology 500 Gene List - Chinese (Simplified)
- TruSight Oncology 500 and TruSight Oncology 500 High-Throughput
- Data analysis and reporting for the TruSight Oncology 500 portfolio
- Chinese (Simplified)
- TruSight Oncology 500 portfolio
Support Documentation
Specifications
- Area of interest Cancer research, Drug discovery & development, Immuno-oncology research, Immunology
- Assay time 3–4 days from sample input to final results
- Automation capability Liquid handling robot(s)
- Cancer type Pan-cancer, Solid tumor
- Content specifications Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included. The included HRD panel † includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics. † TruSight Oncology 500 v2 is not available for sale in Japan
- Hands on time ~3.25 hrs for automated workflow ~5-7 hrs for manual workflow
- Input quantity 30 ng DNA (as low as 10 ng), 40 ng RNA (as low as 20 ng)
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research ModeNovaSeq 6000 SystemNovaSeq 6000Dx in Research ModeNovaSeq X Plus SystemNovaSeq X System
- Key use Targeted analysis, Tumor immunogenicity analysis
- Method Target enrichment, Targeted DNA sequencing, Targeted RNA sequencing
- Multiplexing NextSeq 550/Dx: 8 samples/run. NexSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples. NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples. NovaSeq X/X+: 1.5 B 32 samples, 10B 192 samples, 25B 480 samples.
- Nucleic acid type DNA, RNA
- Specialized sample types FFPE tissue
- Species category Human
- Technology Sequencing
- Variant class Copy number variants (CNVs), Gene fusions, Genomic instability score (GIS), Insertions-deletions (indels), Loss of heterozygosity (LOH), Microsatellite instability (MSI), Multi-nucleotide variants (MNVs), Single nucleotide variants (SNVs), Somatic variants, Transcript variants, Tumor mutational burden (TMB)
Components
- 20007002 — TruSight Oncology Library Prep (-20C)
- 20007003 — TruSight Oncology Library Prep (4C)
- 20006937 — TruSight Oncology UP Index Primers
- 20018492 — TruSight UMI Adapter v1
- 20026144 — TruSight Oncology Enrich(-20C)New cnfig
- 20026143 — TruSight Oncology Enrich(4C) New cnfig
- 20026138 — TruSight Oncology 500 DNA Probes
- 15057929 — NextSeq 500 High output reagent Carr.V2 (300 cyc)
- 20022408 — NextSeq High Output Flow Cell V2.5
- 15057941 — NextSeq 500 buffer cartrige v2
- 15058251 — NextSeq accessory box V2
links and documants
- Datasheet url Click here
- Source url Click here
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TruSight Oncology 500 DNA Kit for Use with NextSeq plus Velsera interpretation report (16 indexes, 48 Samples)
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