TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)
Vendor: Illumina
SKU: 20020597
In stock
Includes RiboZero beads to deplete rRNA from human, mouse, and rat, PCR master mix to transcript RNA into cDNA , and reagents for preparing 48 samples. Purchase index adapters and Purification Beads separately.
TruSeq Stranded Total RNA provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.
Documents
- Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
- TruSeq Stranded mRNA and Total RNA
Support Documentation
- TruSeq Stranded Total RNA Support - Documentation & Literature Documentation
- TruSeq Stranded Total RNA Consumables & Equipment Documentation
- TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
- TruSeq Stranded Total RNA Documentation
- TruSeq Stranded Total RNA Reference Guide Documentation
- TruSeq Stranded Total RNA Checklist Documentation
- All TruSeq Stranded Total RNA Support
Specifications
- Area of interest Cancer research, Complex disease genomics, Drug discovery & development, Microbiology
- Assay time 11.5 hr
- Automation capability Liquid handling robot(s)
- Content specifications Captures coding RNA plus multiple forms of non-coding RNA
- Hands on time 5.5 hr
- Input quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
- Key use Gene expression analysis, Whole-transcriptome analysis
- Mechanism of action Bead-based rRNA depletion, cDNA synthesis, and PCR
- Method Whole-transcriptome sequencing
- Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together, High-throughput kits: Prepare 96 indexed samples using either combinatorial dual indexes or unique dual indexes
- Nucleic acid type RNA
- Specialized sample types FFPE tissue
- Species category Human, Mouse, Rat
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Gene fusions, Novel transcripts, Single nucleotide polymorphisms (SNPs), Transcript variants
links and documants
- Datasheet url Click here
- Source url Click here
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TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)
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