TruSeq Stranded Total RNA Library Prep Gold (96 Samples)
Vendor: Illumina
SKU: 20020599
In stock
Includes RiboZero beads to deplete rRNA and mitochondrial rRNA from human, mouse, and rat, PCR master mix to transcript RNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters separately. Purchase index adapters and Purification Beads separately.
TruSeq Stranded Total RNA provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.
Documents
- Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
- TruSeq Stranded mRNA and Total RNA
Support Documentation
- TruSeq Stranded Total RNA Support - Documentation & Literature Documentation
- TruSeq Stranded Total RNA Consumables & Equipment Documentation
- TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
- TruSeq Stranded Total RNA Documentation
- TruSeq Stranded Total RNA Reference Guide Documentation
- TruSeq Stranded Total RNA Checklist Documentation
- All TruSeq Stranded Total RNA Support
Specifications
- Area of interest Cancer research, Complex disease genomics, Drug discovery & development, Microbiology
- Assay time 11.5 hr
- Automation capability Liquid handling robot(s)
- Content specifications Captures coding RNA plus multiple forms of non-coding RNA
- Hands on time 5.5 hr
- Input quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
- Key use Gene expression analysis, Whole-transcriptome analysis
- Mechanism of action Bead-based rRNA depletion, cDNA synthesis, and PCR
- Method Whole-transcriptome sequencing
- Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together, High-throughput kits: Prepare 96 indexed samples using either combinatorial dual indexes or unique dual indexes
- Nucleic acid type RNA
- Specialized sample types FFPE tissue
- Species category Human, Mouse, Rat
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Gene fusions, Novel transcripts, Single nucleotide polymorphisms (SNPs), Transcript variants
links and documants
- Datasheet url Click here
- Source url Click here
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TruSeq Stranded Total RNA Library Prep Gold (96 Samples)
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