TruSeq Stranded Total RNA Library Prep Globin (48 Samples)
Vendor: Illumina
SKU: 20020612
In stock
Includes RiboZero beads to deplete rRNA and globin mRNA from blood samples, PCR master mix to transcript RNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters and Purification Beads separately.
TruSeq Stranded Total RNA with Ribo-Zero Globin delivers a clear and comprehensive view of the transcriptome from blood-derived total RNA, with fast, efficient sequencing library preparation.
Documents
- TruSeq Stranded mRNA and Total RNA
- Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Support Documentation
- TruSeq Stranded Total RNA Sample Preparation User-Supplied Consumables and Equipment Documentation
- TruSeq Stranded Total RNA Support - Documentation & Literature Documentation
- TruSeq Stranded Total RNA Consumables & Equipment Documentation
- TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
- Sequencing Library qPCR Quantification Guide Documentation
- Illumina Experiment Manager User Guide Documentation
- TruSeq Stranded Total RNA Dual Index Product Insert Documentation
- TruSeq Stranded Total RNA Documentation
Specifications
- Area of interest Complex disease genomics, Drug discovery & development
- Assay time 11.5 hr
- Automation capability Liquid handling robot(s)
- Content specifications Captures coding RNA plus multiple forms of non-coding RNA
- Hands on time 5.5 hr
- Input quantity 0.1 – 1 ug high-quality purified total RNA from blood
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
- Key use Gene expression analysis, Whole-transcriptome analysis
- Mechanism of action Bead-based rRNA depletion, cDNA synthesis, and PCR
- Method Whole-transcriptome sequencing
- Multiplexing High-throughput kits: Prepare 96 indexed samples using either combinatorial dual indexes or unique dual indexes, Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together
- Nucleic acid type RNA
- Specialized sample types Blood
- Species category Human, Mouse, Rat
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Gene fusions, Novel transcripts, Single nucleotide polymorphisms (SNPs), Transcript variants
Components
- 15032611 — TruSeq Strand. RNA LT Kit48 Sampl.cDNA Synth PCR
- 15032615 — TruSeqSamples-Ribo-Zero Stranded RNA LT Kit 48
- 15035750 — TruSeq Strnd RNA LT kit 48SMP Rbo Zro Gbn
- 20020737 — TruSeq Strnd RNA Core LP Box 1
links and documants
- Datasheet url Click here
- Source url Click here
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TruSeq Stranded Total RNA Library Prep Globin (48 Samples)
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