TruSeq Stranded mRNA Library Prep (48 Samples) 20020594 - Illumina

TruSeq Stranded mRNA Library Prep (96 Samples)

Name: TruSeq Stranded mRNA Library Prep (96 Samples)
Brand: Illumina
SKU: 20020595
Availability: InStock

Vendor: Illumina

SKU: 20020595

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Includes RNA purification beads for upfront polyA capture step, PCR master mix to transcript mRNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters separately. Purfication Beads are required post polyA capture step and are sold separately.

TruSeq Stranded mRNA offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It is compatible with a wide range of samples.

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Support Documentation

Specifications

Area of interest Cancer research, Complex disease genomics, Drug discovery & development
Assay time ~10.5 hr
Automation capability Liquid handling robot(s)
Content specifications Captures the coding transcriptome with strand information
Hands on time ~4.5 hr
Input quantity 0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails)
Instruments MiSeq SystemNextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
Key use Coding transcriptome analysis, Gene expression analysis
Mechanism of action Oligo-dT beads capture polyA tails
Method mRNA sequencing
Multiplexing 1–96
Nucleic acid type RNA
Specialized sample types Not FFPE-compatible
Species category Bovine, Human, Mammalian, Mouse, Rat
Sub category Library preparation kits
Technology Sequencing
Variant class Gene fusions, Novel transcripts, Single nucleotide polymorphisms (SNPs), Transcript variants

links and documants

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