TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples) 20020492 - Illumina

TruSeq RNA Library Prep for Enrichment (48 Samples)

Name: TruSeq RNA Library Prep for Enrichment (48 Samples)
Brand: Illumina
SKU: 20020189
Availability: InStock

Vendor: Illumina

SKU: 20020189

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Includes reagents for preparing 48 libraries. Purchase index adapters, enrichment panel, and enrichment reagents separately.

TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.

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Specifications

Area of interest Complex disease genomics, Immuno-oncology research
Assay time ~2 days
Automation capability Liquid handling robot(s)
Content specifications Captures the coding transcriptome/RNA exome
Hands on time ~11 hr
Input quantity 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples
Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
Key use Coding transcriptome analysis, Gene expression analysis
Mechanism of action Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.
Method Exome sequencing, mRNA sequencing
Multiplexing Up to 24 single, 96 combinatorial (CD) dual
Nucleic acid type RNA
Specialized sample types FFPE tissue, Low-input samples
Species category Human
Sub category Library preparation kits
Technology Sequencing
Variant class Novel transcripts, Single nucleotide polymorphisms (SNPs), Transcript variants

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