TruSeq RNA Enrichment (12 enrichments)
Vendor: Illumina
SKU: 20020490
In stock
Sufficient for 12 enrichment reactions when paired with TruSeq RNA Library Prep for Enrichment. Purchase library prep, index adapters, and probe panels separately.
TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.
Documents
- TruSeq RNA Exome Data Sheet
- Evaluating RNA Quality from FFPE Samples
- Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Support Documentation
Specifications
- Area of interest Complex disease genomics, Immuno-oncology research
- Assay time ~2 days
- Automation capability Liquid handling robot(s)
- Content specifications Captures the coding transcriptome/RNA exome
- Hands on time ~11 hr
- Input quantity 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
- Key use Coding transcriptome analysis, Gene expression analysis
- Mechanism of action Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.
- Method Exome sequencing, mRNA sequencing
- Multiplexing Up to 24 single, 96 combinatorial (CD) dual
- Nucleic acid type RNA
- Specialized sample types FFPE tissue, Low-input samples
- Species category Human
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Novel transcripts, Single nucleotide polymorphisms (SNPs), Transcript variants
links and documants
- Datasheet url Click here
- Source url Click here
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TruSeq RNA Enrichment (12 enrichments)
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