Tribo™ Reverse Transcription Kit is optimized to synthesize first-strand cDNA for PCR or qPCR. The optimal first-strand cDNA synthesis temperature is 50°C. The reverse transcription reaction at higher temperatures improves cDNA yields for difficult RNA transcription. This pre-mixed 5X concentrated solution includes all necessary components for first-strand synthesis. With the mixture of 5X RT buffer, dNTPs, MgCl2, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, and Reverse Transcriptase, the first-strand cDNA synthesis process is greatly simplified.
Applications:
This kit can be used for first-strand cDNA synthesis.
Key Features:
- Easy-to-use pre-mixed package (All in One)
- High fidelity, yield, and sensitivity
- Broader linear range
- Complete cDNA synthesis guaranteed
- Robust and high throughput
- Work with difficult sequences
Kit Contents:
5x RT reagent: 0.2mL
The kit contains RT buffer, dNTPs, DTT, RNase inhibitor, Primer mixer, and Reverse Transcriptase. The reagent is enough for 50 RT reactions in 20μL.
Storage Conditions:
The kit is shipped on ice. All components should be stored at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receiving.
Research use only.
Documents
References
1. Ramos-León, J., Valencia, C., Gutiérrez-Mariscal, M., Rivera-Miranda, D.-D.-A., García-Meléndrez, C., & Covarrubias, L. (2023, March 09). The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism. bioRxiv. https://www.biorxiv.org/content/10.1101/2023.03.09.531979v1
2. Ramos-León, J., Valencia, C., Gutiérrez-Mariscal, M., Rivera-Miranda, D. A., García-Meléndrez, C., & Covarrubias, L. (2024). The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism. Experimental cell research, 437(1), 113965. https://doi.org/10.1016/j.yexcr.2024.113965
3. Arjavalingam, A. (2022). Modeling Neuronal Circadian Rhythms in Major Depressive Disorder using Human Induced Pluripotent Stem Cells. UC San Diego. https://escholarship.org/uc/item/9kf1j00p
Research use only.
