Tribioscience’s Mouse IL-5 Fast ELISA is designed to quantitatively detect mouse IL-5 levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 15 to 1000 pg/mL. The levels of mouse IL-5 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-5 protein.
Typical Data
This standard curve (R2=0.9997) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of mouse IL-5 is typically 7.6 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant mouse IL-5.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
Mouse IL-5 Microplate
TBS3033A
96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for mouse IL-5.
Return unused wells to the foil pouch. Reseal along entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Mouse IL-5 Standard
TBS3033B
30 µL of Recombinant mouse IL-5 protein (50 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3033C
2.2 mL of Biotin-mouse IL-5 antibody.
May be stored for up to
3 months at 2-8 °C.*
Detection B
TBS3033D
300 µL of Streptavidin-HRP.
Assay Diluent
TBS3033E
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
