Tribo™ DNase I Solution (1 mg/mL)

DNase I (RNase-Free) is an endonuclease that efficiently hydrolyzes double- (ds) or single stranded DNA to a mixture of short oligo- and mononucleotides. In the presence of Mg2+, cleavage of each strand of a dsDNA substrate proceeds independently. In contrast, in the presence of Mn2+, the enzyme cleaves both strands of DNA at approximately the same site to generate molecules with blunt ends or 1- or 2-base overhangs that can be blunted with T4 DNA Polymerase.

The DNase I Solution is provided at a concentration of 1mg/mL (2000U/mL). 10X Reaction Buffer and Stop Solution are included in the kit.

 

Applications

• Elimination of the DNA template following in vitro RNA synthesis with T7 Phage RNA Polymerase.
• Characterization of DNA: protein interactions by one of “DNase I footprinting”.
• Treatment of RNA prior to RT-PCR.
• Radiolabeling of DNA by nick translation.

 

Storage Conditions

The kit is shipped on blue ice. Keep all components at -20℃ for long-term storage.
Shelf life of 12 months after receiving.

 

Related Products

1. Cell RNA Isolation Kit (TBS6001)
2. Blood RNA Isolation Kit (TBS6002)
3. Tissue RNA Isolation Kit (TBS6003)
4. Mouse Tail DNA Extraction (TBS6005)
5. Tissue DNA Extraction (TBS6006)

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)