TriboTM 2x Fast Sybr Green qPCR Master Mix with different ROX level and package size provides a convenient choice suitable for different qPCR instruments. It is a 2x concentrated premix containing Taq DNA polymerase, dNTPs, Mg2+, SYBR Green I dye (detection), ROX reference dye and PCR buffer components. The proprietary chemical modifications of the enzyme make PCR hot start activation much faster (less than 1 min) than standard qPCR. It does not need 10 min activation in the standard real-time qPCR amplification, which confers a significant reduction in non-specific PCR amplification (please see the diagram in the data sheet). As the enzyme buffer system is optimized for real time PCR, the product offers high amplification efficiency and high detection sensitivity in real-time PCR.
Applications:
This kit can be used for real-time PCR amplification.
Key Features:
Fast: Only 1min, Not need 10 or 5 min for Enzyme activation.
Multiple choices: 2X Master Mix with different ROX level can be suitable for different instruments.
Kit Contents:
Kit Catalog
Description
Kit Size
qPCR Instrument
TBS4001R-1.25
2x Fast SyBr Green qPCR Master Mix ROX
1 x 1.25 mL
ABI® PRISM® 7000, 7700, 7900HT, ABI® 7300 qPCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™.
Gao, J., Sikal, A., Hankin, R., Zheng, Y., Sterling, E., Chan, K., & Yao, Y. (2025). Extracellular Vesicles from Regenerating Skeletal Muscle Mitigate Muscle Atrophy in an Amyotrophic Lateral Sclerosis Mouse Model. Cells, 14(6), 464. https://doi.org/10.3390/cells14060464
Research use only.
Specifications
Storage
The kit is shipped on ice and should be stored at -20°C for long-term storage