The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table. 1).
Applications
Routine cloning of DNA up to 200 kb.
Benefits
High transformation efficiency with clones of all sizes, including BAC clones (Table. 1). lacZΔM15 for blue/white screening of recombinants. Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA. Endonuclease minus (endA1) to ensure high yields of DNA. Recombination minus (recA1) for greater stability of large cloned inserts. DNA TransforMax EC100 Chemically Competent E. coli TransforMax EC100 Electrocompetent E. coli pUC19 1.4 × 108 1.4 × 1010 8.1-kb Clone 1.3 × 107 Not tested 13.1-kb Clone 4.3 × 106 1.3 × 109 23.1-kb Clone 9.2 × 105 3.0 × 108 145-kb BAC Clone Not tested 7 × 107 13.1-kb clone directly from a ligation reaction 2.2 × 105 2.1 × 107
Table. 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µL of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µL aliquot from a standard 10-µL ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.
