Aflatoxins are a class of structurally similar mycotoxins produced by Aspergillus species. Currently, 17 different types of aflatoxins have been discovered, including aflatoxins B1 and B2 (produced by A. flavus and A. parasiticus), aflatoxins G1 and G2 (produced by A. parasiticus), and aflatoxins M1 and M2, metabolites of B1 and B2 respectively. Total Aflatoxin, the most common form of aflatoxin that originated in naturally contaminated food, has acute toxicity to animals and has been classified as a Group 1 carcinogen by IARC of World Health Organization.
Tribioscience’s Total Aflatoxin ELISA Kit can quickly, sensitively, and accurately determine the presence of total aflatoxin in plants, herbs, foods, and animal feeds. It provides a vital tool to prevent the consumption of food tainted with this toxic chemical.
Kit Components
Part
Part#
Description
Storage of Opened/Reconstituted
Pre-coated Microplate
TBS21131A
96 well polystyrene microplate (12 strips of 8 wells) coated with an antibody specific for total aflatoxin.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Total Aflatoxin Standard
TBS21131B
80 µl of total aflatoxin (80ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS21131C
80 µl of HRP- aflatoxin conjugate (100x).
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS3000D
25 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 1 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
