TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression
Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.
Table 1. Competent Cells for Phage Display libraries
Cell Lines
Transformation Efficiency (cfu/µg pUC DNA)
Cloning Methylated DNA
BAC, Cosmid Cloning
Blue/White Screening
TG1 Electrocompetent
>4 × 1010
YES
NO
YES IPTG induction required
SS320 (MC1061 F') Electrocompetent
>4× 1010
YES
NO
YES IPTG induction required
MC1061 F-Electrocompetent
>4× 1010
YES
NO
YES IPTG induction required
ER2738 Electrocompetent
>2 × 1010
YES
NO
YES IPTG induction required
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Key features
Perfect for antibody phage display library creation
The highest efficiency TG1 competent cells available.
Create larger libraries; speed discovery
Bulk custom dispensing available with all of our phage display cells
Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
