Tet System Approved FBS

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Features

• All-in-one vector design provides all the power of Tet-On 3G technology in a single vector, making inducible expression as easy as constitutive expression
• Low background and high maximum induced expression
• High sensitivity to doxycycline: near maximum expression at 10 ng/ml doxycycline
• Inducible expression in a broad range of tissues, because the Tet-On 3G transactivator is expressed from the human PGK promoter
• High-titer lentivirus packaging system provided with Lenti-X Tet-One systems

Applications

Tightly controlled inducible expression in mammalian cells

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• Tet-One Inducible Expression System Components List
• pTetOne Vector Information
• pTetOne Vector Sequence in GenBank Format
• pTetOne-Luc Control Vector Sequence in GenBank Format

Kit Components

linear selection markers for resistance to puromycin and hygromycin, either of which can be cotransfected with the pTetOne Vector to obtain stable clones