Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha, mCherry)

The Tet-On 3G Inducible Expression System (EF1alpha, mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a red fluorescent protein (mCherry) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-mCherry Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the mCherry marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pEF1a-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a human elongation factor 1 alpha (EF1α) promoter, allowing expression of the protein in a variety of mammalian cells (such as hematopoietic or stem cells) without the transgene silencing associated with CMV promoters. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Features

• Equivalent co-regulated and simultaneous expression of two genes
• Indirectly monitor expression of a target gene via co-induced expression of a bright green or red fluorescent protein
• Same tight control as all Tet-On 3G systems
• Highly sensitive to doxycycline
• You may alternatively be interested in Tet-On 3G Systems that express two genes from a single IRES bicistronic transcript

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• Tet-On 3G Bidirectional Inducible Expression System (with mCherry) Components List
• pTRE3G-BI-mCherry Vector Sequence in GenBank Format
• pTRE3G-BI-mCherry Vector Information
• pTRE3G-BI-Luc Control Vector Information
• pTRE3G-BI-Luc Control Vector Sequence in GenBank Format
• pCMV-Tet3G Vector Information
• pCMV-Tet3G vector sequence file in .gb format
• Components

Required Products

⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.