Terra™ qPCR Direct TB Green® Premix

Terra qPCR Direct TB Green Premix is a 2X master mix designed specifically for real-time PCR with TB Green chemistry. In addition to TB Green dye, this mix contains Terra qPCR Direct Polymerase, a novel enzyme developed for optimal amplification from crude or dirty templates; it’s perfect for amplifying short DNA targets (up to 2 kb), regardless of GC content or template purity. The premix also contains a monoclonal antibody that suppresses polymerase activity up to 98°C, allowing automatic hot start PCR.

Features

• Perform real-time PCR with crude tissue extracts or dirty samples—no template purification necessary
• Easily amplify GC-rich targets
• Convenient 2X master mix contains TB Green dye
• Optimized for use with the laser-, lamp-, or LED-based real-time instrument of your choice
• Automatic hot start

Applications

Perform direct real-time PCR from crude extracts and dirty samples, without having to purify your template

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• Library Quantification Kit Components List