TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat.# RR420L and RR420W are larger package sizes [5 ml and 25 ml (5 x 5 ml), respectively]. Each provide convenient larger packaging and are supplied with a single, separate vial of ROX Reference Dye.
Features
Lower Cq values as a result of fast extension rates
Use with broad range of amplicon sizes, even over 500 bp
Convenient 2X master mix is excellent for high-speed qPCR
High sensitivity: detects fewer than 100 copies
Hot-start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
Applications
TB Green qPCR using TB Green and ROX Reference Dye Gene expression analysis Genotyping by real-time PCR High-throughput real-time PCR Amplification of long products
