TB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration premixed with TB Green at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent also contains Tli RNaseH, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as template. This product, with a modified buffer composition, offers a higher reaction specificity than that by TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat.# RR420A). With inhibition of non-specific amplifications, which interfere with quantitative determination, accurate assays over a wide range are possible. A combination of this buffer and Takara Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, allows highly reproducible and reliable real time PCR analyses. Cat. #RR820L and RR820W are larger package sizes [5 ml and 25 ml (5 x 5 ml), respectively] of TB Green Premix Ex Taq II (Tli RNase H Plus) (#RR820A). Each of these includes a separate vial of ROX Reference Dye.
Features
High specificity minimizes primer-dimer formation and nonspecific amplification
Excels with GC-rich targets
2X master mix provides convenience
High sensitivity: detects fewer than 100 copies
Compatible with all commonly used qPCR instruments
Applications
Gene expression analysis qPCR Genotyping Suppression of primer dimers or off-target products
