TaKaRa POD Conjugate is a reagent designed for immunohistochemical staining of paraffinembedded tissue sections. This product consists of an amino acid polymer combined with peroxidase and Fab' fragments of a secondary antibody. It is supplied at a concentration suitable for immediate use, in MOPS (3-(N -Morpholino) propanesulfonic acid) buffer (pH6.5) containing stabilizing protein and antibiotics. This product provides clear, highly sensitive results with minimal background staining, and is simpler and easier to use than the streptavidin-biotin method. This product is available in two different formats, "For Mouse Tissue" (for mouse tissue sections) and "For Tissue" (for tissue sections from other mammalian sources). Three specific versions are available in each format, based on the source species of the primary antibody. "Anti Mouse", "Anti Rat" and "Anti Rabbit" versions are available "For Mouse Tissue", and "Anti Mouse", "Anti Rabbit" and "Anti Goat" versions are available "For Tissue". The TaKaRa POD Conjugate Set Anti Mouse, For Mouse Tissue (Cat. #MK200) is unique because it may be used with a mouse primary antibody on mouse tissue. It consists of Blocking Reagents A and B and TaKaRa POD Conjugate Anti Mouse, For Mouse Tissue. The "For Tissue" products can be used with tissue sections from various species, but it is important to determine in advance the appropriate conditions for each tissue and primary antibody.
Features
Do not react with endogenous immunoglobulins (Ig)
Not affected by endogenous biotin
No need for a blocking reaction or addition of an enzyme-conjugated reagent that reacts with the secondary antibody
