Takara EpiTaq HS (for bisulfite-treated DNA) is a DNA polymerase optimized for PCR amplifications using bisulfite-treated DNA containing uracil as template. PCR reactions with bisulfite-treated DNA often pose particular technical challenges for conventional enzymes. TaKaRa EpiTaq HS and the reagents contained in this kit, however, allow adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiencies and specificities, facilitating excellent amplifications even for targets that previously were impossible to amplify.
Features
Superior amplification of bisulfite-treated DNA
Efficient amplification of a range of amplicons ranging from short (150 bp) to long (1,000 bp)
EpiTaq HS enzyme inhibits non-specific amplification and primer-dimer formation
Amplified product can be used directly for TA cloning applications
Applications
Methylation analysis of CpG Islands Amplification of bisulfite-treated DNA, including DNA templates that contain uracils DNA amplification of AT/GC rich templates
