The column package allows you to combine affinity with size exclusion chromatography to obtain highly pure his-tagged proteins.
Contains a HisTrap HP column 1 mL prepacked with Ni Sepharose High Performance (HP) affinity resin for preparative purification of his-tagged proteins.
You achieve final purity with HiPrep 16/60 Sephacryl S-200 HR size exclusion chromatography column, which removes any remaining trace of impurities or closely related substances and imidazole.
Includes instructions with guidance and tips showing you how to get started.
Nickel ion chelating groups for efficient capture of your his-tagged proteins
Ni Sepharose HP affinity resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups. Ni Sepharose HP has a binding capacity that exceeds 40 mg/mL, resulting in high protein recovery.
High final protein purity through size exclusion chromatography
The size exclusion column is prepacked with Sephacryl S-200 HR chromatography resin, which has a broad fractionation range of Mr 5 000 to ~ 250 000 for globular proteins. The high sample capacity of the HiPrep column lets you load samples of up to 5 mL.
