T4 RNA Ligase

T4 RNA Ligase catalyzes the formation of phosphodiester bonds between singlestranded nucleic acids with 5'-phosphate and 3'-hydroxyl termini. The enzyme requires ATP for activity. The rate of activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation and very low for DNA-DNA ligation. T4 RNA Ligase is supplied in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol.

Features

• Ligation of single-stranded RNA or DNA
• 3'-end labeling of single stranded RNA or DNA

Applications

Ligation of single-stranded RNA or DNA
3'-end labeling of single stranded RNA or DNA

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)