T-Vector pMD™20

T-Vector pMD20 is a linearized vector with a single 3'-terminal thymidine at both ends. The T-overhang ends at the cloning site improves the efficiency of ligation of PCR products which contain A-overhangs at 3'-ends. T-Vector pMD20 is derived from a pUC19 backbone with modifications in the coding sequence to reduce the false positive rate and improve the positive colonies screened by blue/white color on ampicillin/IPTG/X-Gal LB plates. In addition, an SP6 promoter has been inserted upstream of the multiple cloning site. Therefore, the inserted DNA fragments can be transcribed by SP6 RNA Polymerase (Cat. #2520). T-Vector pMD20 is supplied with a Control Insert (500 bp) DNA for positive control reactions.

Features

• Concentration: 50 ng/µl
• Amount: 1 µg
• Vector size: pMD20 2,736 bp; pMD19 (Simple) 2,692 bp

Applications

TA cloning
PCR cloning
SP6 expression (pMD20 only)

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• T-Vector pMD20 (Linear) Vector sequence file in GenBank format