Synthetic siRNA Quantitation Core Kit is designed to quantify synthetic siRNA when used in combination with realtime PCR analysis, and is used to assay the residual amount of the administered siRNA in total RNA samples extracted from cells, tissues, blood, and other specimens.
Features
This kit is designed for siRNA quantitation of molecules containing 3' d(TT) overhangs.
For synthetic siRNA with 3ʼoverhangs other than d(TT), prepare a specific oligo dT primer containing the modified sequence.
This product does not work with synthetic siRNAs that carry no 3’ overhangs or with RNA 3’ overhangs.
Applications
siRNA quantitation in total RNA with intercalating green dye Real Time PCR (qPCR) Low-copy-number siRNA quantitation
