Superose™ 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range M r ~ 5K to ~ 5M.
- Wide fractionation range: optimized to purify large biomolecules, including membrane proteins, protein complexes, and DNA fragments
- Very high resolution: small bead size and a narrow particle size distribution provide high resolution, for high protein purity
- High flow rates: rigid beads give excellent pressure/flow properties
- Enhanced performance: improved resolution and runtime compared to predecessor
“Increase”: Cytiva's new generation of size exclusion chromatography columns
Superose™ 6 Increase is a next-generation, agarose-based matrix for size exclusion chromatography. With smaller and more rigid beads than their predecessors, Superose™ 6 Increase columns deliver higher resolution protein purification in shorter runtimes.
Fractionation of large proteins and protein complexes
Suitable for both preparative and analytical applications, Superose™ 6 Increase SEC columns are the first choice for the characterization of large proteins and protein complexes. They can also be used the study of protein-protein interactions, and as a screening tool to explore the molecular weight distribution of an unknown sample.
Superose™ 6 and Superdex™ 200 analysis ranges are complementary
Superose™ 6 Increase is designed for separation of protein complexes and other very large molecules, including those with molecular weights exceeding Mr ~400 000. The optimal fractionation range of Superdex™ 200 Increase is complementary, providing higher resolution for proteins with molecular weights below Mr ~400 000.
Cleaning-in-place (CIP) capabilities
This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.
Regular cleaning can also increase column lifetime.
Choose from 3 column sizes to suit varying application needs
| Column |
Recommended sample volume |
Recommended operating flow |
Efficiency (N/m) |
Applications |
| Superose™ 6 Increase 3.2/300 |
4 to 50 µL |
0.04 mL/min |
>48,000 |
Small-scale preparative purification and analysis of large proteins and other biomolecules, when small sample and buffer consumption is important |
| Superose™ 6 Increase 10/300 GL |
25 to 500 µL |
0.5 mL/min |
> 48,000 |
Standard for small-scale preparative purification and analysis of large proteins and other biomolecules, especially protein complexes |
| Superose™ 6 Increase 5/150 GL |
4 to 50 µL |
0.3 mL/min |
> 42,000 |
Rapid purity check and homogeneity analysis of large proteins and protein complexes |
Feedback on Superose™ 6 Increase SEC columns
"Now I can work on more samples, resulting in higher throughput. I can now purify a protein in 30 minutes instead of an hour." Dr. Tomohiro Nishizawa (Nureki Laboratory). Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Successful use in published research
Researchers use these pre-packed columns for a variety of applications, such as:
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