Stellar Electrocompetent Cells are an E. coli HST08 strain that provides high transformation efficiency, good reproducibility, and blue/white screening when paired with pUC plasmid vectors. These cells are specially made for transformation using the electroporation method. Stellar Electrocompetent Cells lack the gene cluster which digests foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning of methylated DNA, in addition to construction of genomic libraries and longer-length genomic libraries. A pUC19 vector is provided as well as SOC Medium.
Features
Cloning of large fragments
cDNA library construction
Genomic library construction
Long-length genomic library construction
Applications
Cloning of large fragments cDNA library construction Genomic library construction Long-length genomic library construction
