SMARTer Target RNA Capture for Illumina is used to generate cDNA from enriched RNA transcripts. Target-specific biotinylated DNA probes designed by the user are hybridized to transcripts of interest and the resulting RNA-DNA hybrids are captured with Capture Beads. This kit takes advantage of the sensitivity of the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing to generate high-quality, full-length cDNA from these enriched transcripts. The kit was tested with 10 ng of total RNA, and has been shown to perform cDNA synthesis from enriched transcripts derived from as little as 100 pg purified total RNA. cDNA libraries generated by this kit have been tested for compatibility with Illumina sequencing platforms. This method does not require additional rRNA removal methods or kits.
Features
Inputs as low as 10 ng of purified total RNA
Streamlined workflow for specific enrichment of transcripts of interest
Generate Illumina-ready, full-length sequencing libraries from the enriched DNA
Ligation-independent sequencing adapter addition for library preparation
Applications
Sequencing library preparation for targeted RNA-seq from purified total RNA Illumina-specific NGS sequencing library production Detection of rare gene fusion events at low sequencing depth
