The SMARTer smRNA-Seq Kit for Illumina is used to generate small RNA-seq libraries for sequencing on Illumina platforms. This kit was developed to work directly with total RNA or enriched small RNA (including microRNA), for inputs ranging from 1 ng–2 μg. By incorporating features of the SMARTer Stranded RNA-Seq kits, including our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, and primers that include locked nucleic acids (LNAs), this kit allows users to analyze a wide range of smRNA species and generate sequencing libraries of considerable complexity from as little as 1 ng of input material. Illumina adapter and index sequences are incorporated in a ligation-free manner during library amplification, ensuring that diverse smRNA species are represented with minimal bias.
Features
Analysis of diverse small RNA species—Sequencing library prep of small RNAs ranging in size from 15–150 nt
Flexible sample inputs—Start from 1 ng–2 µg of total RNA or enriched small RNA
Illumina-ready sequencing libraries—Ligation-free incorporation of Illumina adapters and indexes for sample multiplexing
Quick, single-tube workflow—Generate sequencing libraries in ~3 hours (not including validation and post-PCR size-selection steps)
Applications
Illumina-ready sequencing library preparation, specifically for small RNAs ranging in size from 15–150 nt
