SMARTer® Human scTCR a/b Profiling Kit

The SMARTer Human TCR a/b Profiling Kit enables users to analyze T-cell receptor (TCR) diversity from single T cells that have been sorted into a 96-well plate. As the name suggests, the kit can be used to generate data for both alpha- and beta-chain diversity. The kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of TCR transcripts. Included in the kit are primers that incorporate Illumina-specific adaptor sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms.

Features

• Flexible workflow: Illumina-ready libraries from FACS or manually sorted single cells
• Ease of use: optimized indexing allows for pooling 96 cells into twelve libraries which can be further multiplexed for running in a single flow-cell lane
• Sensitivity: RACE-based approach allows for the detection of low-abundance TCR variants
• Specificity: full-length reads, with the majority of reads on target and accurate pairing information

Applications

Human TCR repertoire analysis in single cells (TCR-alpha and TCR-beta subunits)
TCR alpha-beta pairing determination in single cells

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• SMARTer Human scTCR a/b Profiling Kit Components List