The SMARTer Human BCR IgG IgM H/K/L Profiling Kit enables users to analyze B-cell receptor (BCR) repertoires from total RNA samples. This kit can generate libraries from 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) and 1 to 100 ng of total RNA from B cells. This kit may be used for other sample types, but the protocol may need to be modified. The kit can be used to generate data for both heavy and light chains (kappa and lambda) of human immunoglobulin IgG and IgM.
Features
Input: 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) or 1 to 100 ng of total RNA from B cells
UMI-based correction for removal of reads derived from PCR error or duplication and/or sequencing errors
Sensitive and specific clonotype detection with optimized cDNA library generation
Accurate amplification of human IgG subclasses and identification via sequencing in a majority of cases
Optimized PCR pooling strategy for highly sensitive detection of different chains from the same sample
Applications
Human BCR repertoire analysis (heavy and light [kappa and lambda] chains)
