SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) generates strand-specific RNA-seq libraries for Illumina sequencing from 1–1,000 sorted cells or 10 pg–10 ng of purified total RNA. The kit was specifically designed to deliver highly sensitive and reproducible data from single cells while keeping the workflow short and user friendly. The kit does not require additional rRNA removal methods or kits and produces sequencing libraries that retain strand-of-origin information. Enough material is provided with this kit to perform up to 24 reactions.
Features
Simple workflow to generate stranded Illumina sequencing-ready libraries in ~6.5 hours
Appropriate for analysis of 1–1,000 intact mammalian cells or 10 pg–10 ng of low- or high-quality mammalian total RNA
Reproducible, accurate detection of full-length coding and noncoding transcripts from single cells or total RNA
Comparable sensitivity to our gold-standard SMART-Seq mRNA.
Applications
Generation of Illumina sequencing-ready libraries in 7 hours from small quantities of mammalian cells (1–1,000 cells) or total RNA (10 pg–10 ng) Whole transcriptome sequencing with strand-of-origin information
reagents for generating stranded sequencing libraries, including cDNA synthesis and amplification, ZapR-mediated ribosomal RNA depletion, and Illumina-compatible library preparation
Required Products
⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.
