SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs) generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–1 µg inputs of purified total RNA or from 10–1,000 intact cells. The library design featured in this kit adds an 8 nucleotide (nt) unique molecular identifier (UMI) through the reverse-transcription step to mitigate potential PCR bias as well as to provide additional information for transcript quantification. Enough material is provided with this kit to perform up to 96 reactions.
Features
SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs) supports an expanded input range of 250 pg–1 µg total RNA.
SMART-Seq Total RNA Library Prep with ZapR Depletion supports the same expanded input range of 250 pg–1 µg total RNA but does not include the use of UMIs in the library prep
SMART-Seq Total RNA Single Cell (ZapR Mammalian) supports an input range of 1–1,000 cells or 10 pg–10 ng total RNA
Applications
Robust NGS library construction that retains strand information RNA-seq on all Illumina platforms Coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples
