SMART-Seq Total RNA Library Prep with ZapR Depletion generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–1 µg inputs of purified total RNA. The protocol for use with this kit was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. Enough material is provided with this kit to perform up to 384 (4 x 96) reactions.
Features
SMART-Seq Total RNA Library Prep with ZapR Depletion supports an expanded input range of 250 pg–1 µg total RNA.
SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs) supports the same expanded input range of 250 pg–1 µg total RNA and incorporates UMIs in the library prep.
SMART-Seq Total RNA Single Cell (ZapR Mammalian) supports an input range of 1–1,000 cells or 10 pg–10 ng total RNA
Applications
Robust NGS library construction that retains strand information Use for RNA-seq on all Illumina platforms Captures coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples
