The SMART-Seq mRNA Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 24 reactions.
Features
Easy, plate-based workflow—direct input of single cells isolated by FACS or other methods
Single-tube workflows—complete cDNA synthesis and library preparation, in one tube each, for minimization of sample loss, sample mix-up, or other handling errors
Unparalleled sensitivity and reproducibility—get the best performance for single-cell RNA-seq (especially for cells with very low RNA content and for nuclei) with the highest gene detection
Increased sequencing power—include unique dual indexes to allow for the pooling of multiple samples and confident sequencing on the NovaSeq™ system
High library yield—sequence on high-throughput sequencers with ease (such as the NovaSeq system) or confidently save extra library for future runs
Applications
cDNA synthesis from single cells for full-length transcriptome sequencing cDNA outputs from SMART-Seq mRNA Single Cell can be used with SMART-Seq Library Prep (not sold separately; sold as part of SMART-Seq mRNA Single Cell LP) for Illumina sequencing
our patented library preparation kit that incorporates enzymatic fragmentation and stem-loop adapters to construct high-quality, Illumina-compatible libraries from the cDNA synthesized with the core kit
Required Products
⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.
