SMART-Seq mRNA LP (with UMIs) generates oligo(dT)-primed, full-length mRNA-seq libraries with UMIs, providing greater accuracy for quantitative gene expression analysis across samples, while controlling for PCR errors and amplification biases. The chemistry is optimized for use on ultra-low amounts of total RNA (10 pg–100 ng, RIN≥8) or for direct use on multiple intact cells (<1,000 cells). Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index kits (Cat. Nos. 634752–56). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.
Features
Reliability—higher accuracy for gene/transcript counting with UMIs
Superior sensitivity and performance for ultra-low inputs—10 pg–100 ng total RNA, or 1–1,000 cells
Single-tube cDNA synthesis and library prep workflows—minimized sample loss and handling errors
Greater multiplexing—up to 384 libraries with Unique Dual Index Kits (Cat. No. 634752–56)
Complete solution—from sample to data with free, user-friendly Cogent NGS analysis tools
