SMART-Seq mRNA LP generates oligo(dT)-primed, full-length mRNA-seq libraries. The chemistry is optimized for use on ultra-low amounts of total RNA (10 pg–100 ng, RIN ≥ 8) or for direct use on multiple intact cells (< 1,000 cells). Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index kits (Cat. # 634752, 634753, 634754, 634755 & 634756). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.
Features
Superior sensitivity and performance for ultra-low inputs—10 pg–100 ng total RNA, or 1–1,000 cells
High-quality RNA-seq data—identify the highest number of genes; detect gene fusions, isoforms, SNPs
Single-tube cDNA synthesis and library prep workflows—minimized sample loss and handling errors
Greater multiplexing—up to 384 libraries with Unique Dual Index kits (Cat. # 634752, 634753, 634754, 634755 & 634756)
Complete solution—from sample to data with free, user-friendly Cogent NGS analysis tools
Applications
Full-length cDNA synthesis (with SMART-Seq mRNA) and library preparation (with SMART-Seq mRNA LP) from multiple cells or total RNA for transcriptome sequencing cDNA outputs from SMART-Seq mRNA can be used with the SMART-Seq Library Prep kit (sold as part of SMART-Seq mRNA LP) to generate libraries f
