SMART-Seq Mouse BCR (with UMIs) enables users to analyze B-cell receptor (BCR) repertoires from 10 ng–1 µg high-integrity (RIN>7) bulk total RNA samples from spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs) or RNA extracted from mouse whole blood (RNA input ≥100 ng) or lymph nodes. The kit can be used to generate data for both heavy (IgG, IgM, IgA, IgD, and IgE) and light chains (IgK, IgL) of mouse B-cell receptors.
Features
Complete BCR repertoire profiling: includes all heavy (IgG, IgM, IgA, IgD, IgE) and light (IgK, IgL) BCR chains
Supports various inputs: 10 ng–1 μg of high-integrity RNA (RIN>7) from mouse spleen, bone marrow, lymph nodes, whole blood, and PBMCs
Streamlined workflow: primer pools for heavy and light immunoglobulin chains and enzyme premixes provided
Increased accuracy: UMI-based analysis controls for PCR duplicates and errors, correcting for artifacts in clonotype diversity and abundance assessments
Flexible: sequence either the full-length V(D)J region or the CDR3 only and multiplex with up to 384 UDIs
