SMART-Seq Human TCR (with UMIs) enables users to analyze T-cell receptor (TCR) repertoires from bulk RNA samples. This kit can generate libraries from total RNA (10 ng to 1 µg from peripheral blood leukocytes, 20 ng to 200 ng of total RNA obtained from whole blood, or 1 ng to 100 ng T cells) or purified T cells (1,000 to 10,000 cells) and can be used to generate data for both TCR-alpha or TCR-beta subunits. Unique molecular identifiers (UMIs) are incorporated to facilitate PCR error correction and clonotype quantification during data analysis, and unique dual indexes (UDIs) allow users to multiplex more samples and provide confidence when sequencing on patterned flow cells. The kit generates indexed libraries ready for sequencing on Illumina platforms. Easily analyze and visualize your data with Cogent NGS Immune Profiler Software and Cogent NGS Immune Viewer tool.
Features
Use of UMIs to correct for PCR and sequencing errors
Compatibility with UDIs, allowing for greater confidence in sequencing on a patterned flow cell (such as the NovaSeq™ system) and the ability to pool a greater number of samples
Flexibility to sequence on any Illumina instrument
Full-length V(D)J analysis or CDR3-only analysis
Applications
Human TCR repertoire analysis (TCRα and TCRβ subunits)
