SMART-Seq Human BCR (with UMIs) generates oligo(dT)-primed, full-length mRNA-seq libraries for all BCR heavy (IgG/M/D/E/A) and light (IgK/L) chains. The incorporation of unique molecular identifiers (UMIs) provides greater accuracy for quantitative gene expression analysis across samples while controlling for PCR errors and amplification biases. Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index Kits (Cat. # 634752–56). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.
Features
Complete BCR repertoire profiling: includes all heavy (IgG, IgM, IgA, IgD, IgE) and light (IgK, IgL) BCR chains
Supports various inputs: high-integrity RNA from PBMCs (10 ng–1 µg), B cells (1–100 ng), spleen or bone marrow (10 ng), and whole blood (100 ng)
Streamlined workflow: primer pools for heavy and light immunoglobulin chains and enzyme premixes provided
Increased accuracy: UMI-based analysis controls for PCR duplicates and errors, correcting for artifacts in clonotype diversity and abundance assessments
Flexible: sequence either the full-length V(D)J region or the CDR3 only and multiplex with up to 384 UDIs
