Sera-Mag Oligo(dT)-Coated Magnetic Particles enable mRNA isolation and extraction for applications such as RT-PCR, cDNA library construction, cDNA microarrays, affinity purification, primer extension, and subtractive hybridization.
- Encapsulation means no exposed iron and minimized interference with downstream enzymatic applications
- Covalently bound oligo(dT)14 prevents leaching from the particle surface
- Very high, specific poly A+ binding capacity ensures maximum extraction of mRNA
- Fast reaction kinetics increase throughput and precision, also enabling faster movement through viscous solutions
- Uniform, nominal 1 μm diameter provides high surface area and excellent lot-to-lot reproducibility
- Also available as custom-manufactured magnetic beads
Find out which magnetic bead chemistry meets your application requirements in Magbeads 101: A guide to choosing and using magnetic beads and explore ways to optimize your magnetic bead workflow in Scientists guide to magnetic beads
Convenient and efficient mRNA isolation
Eukaryotic mRNA makes up only 1 to 3% of total cellular RNA, so efficient mRNA purification is critical. Sera-Mag Oligo(dT) Magnetic Particles remove 90% or more of mRNA from total RNA with just one extraction. Sera-Mag Oligo(dT) Magnetic Particles bind target mRNA through pairing of the polyadenylated RNA tail found on the 3’ end of mRNA to the covalently bound oligo(dT) groups on the surface of the particles. This binding is easily accomplished using standard hybridization conditions during the mRNA purification process.
Fast magnetic response times and stable physical integrity
The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One layer of magnetite is coated onto this core, while the surface is modified to minimize non-specific binding of proteins. Oligo(dT) groups are then covalently bound to the particle surface. Since the magnetite is encapsulated, there is no exposed iron minimizing interference with downstream enzymatic applications.
Customized magnetic bead solutions that fit seamlessly into your workflows can be developed for specific applications, often saving you time and money. This product can be provided ready-to-use with little or no need for further modification allowing you to focus on your research. To learn more, please visit our custom manufacturing services page.
Have you considered your magnetic separation rack?
Cytiva’s Magnetic Separation Rack 15 mL is a 3D printed, magnetized tube rack with a strong, removable magnetic force and easy pipette access. It facilitates high capture, retention, and release of magnetic beads from various liquid sample media, allowing high-throughput, parallel processing of up to six 15 mL samples.
Find out more about magnetic separation racks
Check out Cytiva Diagnostic Services, helping you build and accelerate the development of smarter molecular and immunodiagnostic assays.
Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.
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