Sephadex G-25 DNA grade SF is suitable for use in preparing spin columns for DNA purification.
For purification of DNA (≥ 10 bases in length) from small molecules by SEC
Tested to ensure reproducibly high recovery of DNA
Used in MicroSpin Columns and NAP Columns
Suitable for researchers who prefer to prepare their own columns for nucleic acid purification
Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.
Sephadex G-50 is a well-established size exclusion chromatography resin for desalting and buffer exchange of biomolecules > 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.
Alternatively, Sephadex G-100 DNA Grade can be used. Sephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA.
All these Sephadex types are therefore highly suitable for the purification of oligonucleotides or small DNA fragments following synthesis or in labeling applications.
Sephadex G-50 DNA Grade, and Sephadex G-100 are also sold separately.
