Sephacryl™ High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale.
Purification over a wide molecular weight range
High reproducibility due to high stability
High recoveries
Well-suited to industrial-scale use
The five chromatography resins available have different porosity ranges. For peptides and small proteins, Sephacryl S-100 HR is the best choice. When fractionating proteins in the molecular weight ranges of 5 × 103 to 2.5 × 105 and 1 × 104 to 1.5 × 106, use Sephacryl S-200 HR and Sephacryl S-300 HR, respectively. Note that these ranges include monoclonal antibodies and serum proteins.
Sephacryl S-400 HR and Sephacryl S-500 HR are recommended for separating polysaccharides and other macromolecules with extended structures, and even small particles such as plasmids.
Differentiating specs (see data file for full specifications):
Application(s)
Sephacryl S-100 HR resin
Sephacryl S-200 HR resin
Sephacryl S-300 HR resin
Sephacryl S-400 HR resin
Sephacryl S-500 HR resin
Fractionation range (M*)
Peptides and small proteins
Antibodies, serum proteins and mid-size proteins
Plysaccharides, macromolecules with extended structures and even small particles such as plasmids
Globular proteins
~ 1 × 103–~ 1 × 105
~ 5 × 103–~ 2.5 × 105
~ 1 × 104–~ 1.5 × 106
~ 2 × 104–~ 8 × 106
--
Dextrans
--
~ 1 × 103–~ 8 × 104
~ 2 × 103–~ 4 × 105
~ 1 × 104–~ 2 × 106
~ 4 × 104–~ 2 × 107
Exclusion limit DNA (base pairs)
--
~ 30 bp
~ 118 bp
~ 271 bp
~ 1078 bp
Prepacked column options
All five Sephacryl HR resins are available in prepacked HiPrep™ Sephacryl HR SEC columns. Each chromatography resin is available in two different prepacked column sizes; 16/60 (120 mL) and 26/60 (320 mL). HiPrep Sephacryl HR SEC columns provide the excellent purification properties of Sephacryl HR resins in a convenient, ready-to-use format. Find the prepacked columns here.
