Prepares samples for SDS-PAGE that are otherwise difficult to analyze because of salts or low protein concentrations
Quantitatively precipitate proteins while leaving interfering substances in solution
Delivers quantitative yield in less than 2 h.
SDS-PAGE Clean-Up Kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyze due to the presence of salts or low protein concentration
SDS-PAGE Clean-Up Kit uses a combination of a precipitant and co-precipitant to quantitatively precipitate the sample proteins while leaving interfering substances in solution. Detergents, salts, lipids, phenolics, and nucleic acids remain soluble while the proteins are pelleted by centrifugation. The precipitate is washed further to remove nonprotein contaminants and centrifuged again. The resultant pellet is resuspended, mixed with SDS-PAGE sample buffer, and heated. The sample is then ready for SDS-PAGE. The procedure can be completed in under 2 h with quantitative yield
