sbeadex Pathogen Nucleic Acid Purification Kit - No Protease; No Dangerous Goods

The sbeadex Pathogen Nucleic Acid Purification Kit without protease and dangerous goods is a cutting-edge solution for fast and reliable isolation of highly purified DNA and RNA from various pathogenic bacteria, viruses and yeasts.

The sbeadex Technology

This kit leverages the power of sbeadex magnetic bead technology. These superparamagnetic particles, coated with sbeadex surface chemistry, employ a novel two-bind / two-wash mechanism that ensures effective removal of impurities from the sample matrix. The second wash buffer in the kit is ethanol-free, eliminating any traces of ethanol that could potentially inhibit downstream reactions. Additionally, the sbeadex technology eliminates the need for a drying step before elution, further enhancing DNA quality.

The inclusion of Proteinase K facilitates efficient cell disruption and nucleic acid release with certain sample material and safeguards its integrity. The Proteinase K enzyme enhances the efficiency of nucleic acid extraction by digestion of proteins in the cell lysate. This addition helps produce higher DNA/RNA yields and improves the stability of extracted nucleic acids.

sbeadex Pathogen Nucleic Acid Purification Kit Competitor A
N Average Cq StDev Average Cq StDev
Clean up sample 12 30.35 0.14 33.80 0.15
Direct spike-in sample 3 31.00 0.44 33.80 0.12

Figure 1: Demonstration of no loss of RNA during the sbeadex Pathogen Nucleic Acid Purification Kit protocol. 500 copies of a 2019-COVID artificial RNA reference material were added to a sample (nasopharyngeal swab) and the sample processed with the sbeadex Pathogen Nucleic Acid Purification Kit. SARS-CoV-2 was detected in this preparation and a directly spiked positive control sample via a RT-qPCR at two separate Biosearch Technologies sites (reaction components described below). The concordant Cq values from both samples demonstrate no loss of sample due to the extraction step.

This is a ready-to-use kit designed to streamline the DNA and RNA purification process, making it an ideal choice for downstream applications such as:

PCR / qPCR

Real Time (RT) PCR / qRT-PCR

Next Generation Sequencing (NGS)

The kit is compatible with a wide range of automation platforms, such as Thermo's KingFisher and Hamilton's Microlab Star or our oKtopure, and can be easily automated on various liquid handlers and purification platforms. It offers high flexibility in reaction volumes, making it suitable for high-throughput purifications.

This kit contains no dangerous goods ensuring an extra level of safety and regulatory compliance.

The sbeadex Pathogen testing kits have been used successfully with:

Pathogen External Control (Artificial Matrix)
Viral Targets
SARS-CoV-2 VTM1
Influenza A H3N2 Swab2
Influenza B Swab2
Novel Influenza A H1N1 Swab2
Respiratory Syncytial Virus (subtype A) Swab2
Respiratory Syncytial Virus (subtype B) Swab2
Adenovirus 4 Swab2
Zika Virus Plasma3
Cytomegalovirus Plasma4
Human Papillomavirus-16 Methanol-preserved cultured human cells containing full-length HPV episomal DNA5
Bacterial Targets
Bordetella pertussis Swab6
Chlamydophila pneumoniae Swab6
Chlamydophila psittaci Swab6
Coxiella burnetii Swab6
Legionella pneumophila Swab6
Mycoplasma pneumoniae Swab6
Mycobacterium tuberculosis Sputum7
Campylobacter jejuni Stool8
Salmonella enteritidis Stool8
Yersinia enterocolitica Stool8
Clostridium difficile Stool8
Chlamydia trachomatis Urine9
Neisseria gonorrhoeae Urine9
Staphylococcus aureus External Run Control10
Other Targets
Candida albicans Positive Extraction Control11
Compatible Matrices Tested Swabs (UTM®/VTM)
Sputum
Saliva
Blood
Plasma
Serum
Urine
Stool
Cerebrospinal Fluid

Table 1: Pathogens that have already been tested using the sbeadex Pathogen Nucleic Acid Purification Kit.

Standards used for testing
AccuPlex™ SARS-CoV-2 Verification Panel - Full Genome, 0505-0168 (LGC SeraCare)
AMPLIRUN® TOTAL RESPIRATORY VIRAL PANEL CONTROL (SWAB), MBTC020 (Vircell S.L.)
AMPLIRUN TOTAL ZIKV/DENV/CHIKV CONTROL (PLASMA), MBTC023 (Vircell S.L.)
AMPLIRUN TOTAL CMV CONTROL (PLASMA), MBTC018-R (Vircell S.L.)
AccuTrak™ HPV Genotype Qualification Panel, QSH701 (2400-0161), (LGC SeraCare)
AMPLIRUN TOTAL ATYPICAL BACTERIAL PNEUMONIA CONTROL (SWAB) MBTC022-R (Vircell S.L.)
AMPLIRUN TOTAL MTB CONTROL (SPUTUM) MBTC013 (Vircell S.L.)
AMPLIRUN TOTAL GASTROINTESTINAL BACTERIAL PANEL CONTROL (STOOL), MBTC021 (Vircell S.L.)
AMPLIRUN TOTAL CT/NG CONTROL (URINE), MBTC003 (Vircell S.L.)
NATtrol™ S. aureus (MRSA) External Run Control, NATSAU(MRSA)-ERCM (Zeptometrix Corporation)
NATtrol Candida/TV Positive Control, NATCTVPOS-BD (Zeptometrix Corporation)

Note: Other sample matrices may also be used with this kit. Further testing on other pathogens and matrices is in progress and this table will be updated accordingly.

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Key features

• Rapid nucleic acid purification from various pathogens
• Contains no dangerous goods, avoiding shipping problems to distinct destinations.
• High-quality sbeadex magnetic bead-based technology
• Ready-to-use kit compatible with most robotic platforms
• Suitable for many downstream applications such as: PCR/qPCR, RT-PCR/qRT-PCR, and NGS

Product Details

The sbeadex Pathogen Nucleic Acid Purification Kit without protease and dangerous goods includes a Lysis Buffer, Binding Buffer, a variety of Wash Buffers, an Elution Buffer, sbeadex particle suspension solution containing EDTA.

This kit does not contain dangerous goods.

Validated for research use only using AccuPlex™ reference material and is compatible with our COVID-19 detection kit.

Choose between 96 or 960 preparations, or enquire for bulk options.

Resources

Application notes

automation sbeadex Pathogen Hamilton

Documents

• Binding Buffer SB
• Binding buffer SB concentrate for Asia Pacific
• Elutions Buffer AMP
• Elutions Buffer AMP for Asia Pacific
• Lysis Buffer SB
• Lysis Buffer SB for Asia Pacific
• PK Solution Protease Solution
• PK Solution Protease Solution for Asia Pacific
• Wash Buffer BN1
• Wash Buffer BN1 concentrate for Asia Pacific
• Wash Buffer PN1 FN1 TN1
• Wash Buffer PN1 FN1 TN1 concentrate for Asia Pacific
• Wash Buffer PN2 FN2 TN2 BLN PLN
• Wash Buffer PN2 FN2 TN2 BLN PLN for Asia Pacific
• sbeadex particle suspension plus EDTA
• sbeadex particle suspension plus EDTA for Asia Pacific
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