DNA purification from plant materials plays a crucial role in various areas of plant research, including genomics, molecular biology, and genetic analysis essential for plant breeding and testing (e.g. GMO testing). The availability of high-quality DNA is essential for reliable results for a variety of applications including PCR, next generation sequencing, genotyping and chip technologies.
The sbeadex™ Mini Plant Kit with No Dangerous Goods is a comprehensive solution designed for extracting DNA from a wide range of plant species. This kit is optimised to efficiently extract and purify DNA from both fresh and lyophilized plant tissue material, leaves, seeds, fruits, pulp, bark and roots.
This kit contains no dangerous goods ensuring an extra level of safety and regulatory compliance.
The Mini Plant Kit is best for small-scale routine purification with a recommended starting material of 5-10 mg for lyophilized tissue or 10-30 mg for fresh tissue. If you’re working with larger scale extractions you can use our sbeadex Maxi Plant Kit.
The DNA purification protocol, especially post optimization with our sbeadex Plant Trial Kit, is easy to automate, allowing for seamless scaling up of sample processing using popular robotic platforms.
The sbeadex Technology
This kit leverages the power of sbeadex magnetic bead technology. These superparamagnetic particles, coated with sbeadex surface chemistry, employ a novel two-bind / two wash mechanism that ensures effective removal of impurities from the sample matrix. The second wash buffer in the kit is ethanol-free, eliminating any traces of ethanol that could potentially inhibit downstream reactions. Additionally, the sbeadex technology eliminates the need for a drying step before elution, further enhancing DNA quality.
sbeadex plant kits have been successfully used with:
Plant species Leaves Seed
Apricot (Prunus armeniaca) X
Barley (Hordeum vulgare) X X
Beet, sugar (Beta vulgaris) X
Canola/oilseed (Brassica napus) X X
Chicory (Cichorium intybus) X
Corn (Zea mays) X X
Cotton (Gossypium) X X
Cucumber (Cucumis sativus) X X
Flax (Linum usitatissimum) X
Grape (Vitis vinifera) X X
Lettuce (Lactuca sativa) X
Muskmelon (Cucumis melo) X X
Onion (Allium cepa) X X
Parsley (Petroselinum crispum) X X
Peach (Prunus persica) X
Pepper (Capsicum annuum) X X
Potato (Solanum tuberosum) X
Rice, Asian (Oryza sativa) X X
Rubber (Hevea brasiliensis) X X
Soybean (Aphis glycines) X X
Sunflower (Helianthus annuus) X X
Tobacco leaves (Nicotiana tabacum) X X
Tomato (Solanum lycopersicum) X X
Wheat (Triticum L.) X X
Typical quantities of starting material are:
5-10 mg lyophilised tissue or 10-30 mg fresh tissue for the sbeadex Mini Plant DNA Purification Kit
10-30 mg lyophilised tissue or 40-120 mg fresh tissue for the sbeadex Maxi Plant DNA Purification Kit
read more
Key features
- Extract high-quality DNA from a wide variety of plant species and materials
- Contains no dangerous goods, ensuring safety and regulatory compliance
- Suitable for small-scale DNA purification
- Suitable for all downstream applications
- Utilises sbeadex magnetic bead-based technology
Product Details
The sbeadex Mini Plant Kit with No Dangerous Goods contains all of the components required to perform small-scale DNA purification from plant tissue. The kit includes 2 wash buffers (concentrate), a lysis buffer, an elution buffer, a binding buffer (concentrate), and a sbeadex particle suspension.
Choose between 96 or 960 preparations, or enquire for bulk options.
As a kit without dangerous goods the binding buffer and wash buffer concentrates contain no hazardous substances.
The kit is compatible with both fresh and lyophilised plant tissue material. The suggested starting material is 5-10 mg lyophilised tissue or 10-30 mg fresh tissue.
We highly suggest optimising your sbeabex DNA purification protocol first with our Mini Plant Trial Kit.
Resources
Application notes
oktopure and sbeadex plant nucleic acid extraction kit
sbeadex mini plant kit and KingFisher 96 instrument
Documents
